Homepage » Green Energy » Green fuels » [NREL] NREL Shows How Cyanobacteria Build Hydrogen-Producing Enzyme

[NREL] NREL Shows How Cyanobacteria Build Hydrogen-Producing Enzyme

0

Results will help researchers improve microbial hydrogen production.

Scientists at the National Renewable Energy Laboratory (NREL) have demonstrated how the cyanobacterium Synechosystis sp. PCC 6803 assembles five different protein subunits to produce a functional hydrogenase enzyme complex. The NREL scientists used genetic tools to perform an in-depth study of Hox hydrogenase, an organometallic enzyme that catalyzes the photo production of hydrogen in Synechocystis, and generated new data on the assembly, maturation, and activity of this important enzyme

To perform this study, NREL generated a series of Hox hydrogenase subunit mutants from a common strain to provide a consistent platform for comparing hydrogenase activity and growth due to the targeted mutations. Genetic analysis of these mutant strains resulted in comprehensive data on how the Hox subunits associate, which revealed the compositions and abundance of the sub-complexes, how the absence of individual Hox subunits or sub-complexes affects enzyme activity, and how genetic mutations affect growth and photosynthesis.

Hydrogen production

This model for Hox hydrogenase association in Synechocystis sp. PCC 6803 depicts the assembly of Hox subcomplexes and complexes. Illustration by Carrie Eckert, NREL

NREL’s work yields new evidence about how the various subunits and sub-complexes of Hox hydrogenase interact with each other to form a functional enzyme, clears up inconsistencies in the literature, and advances understanding of the fundamental processes governing Hox hydrogenase function. It also reveals for the first time the underlying mechanism controlling the assembly and maturation of a complex hydrogenase such as Hox. The previous model for hydrogenase maturation is solely based on a simpler hydrogenase (containing only two subunits) in E. coli, with little knowledge of its more complex counterparts. This newly gained understanding will help guide future modifications to improve Hox hydrogenase activity and increase hydrogen production yields.

Key Research Results

Achievement

NREL demonstrated how the cyanobacterium Synechocystis assembles five different proteins to make a functional hydrogenase enzyme.

Key Result

Genetic analysis of Synechocystis Hox hydrogenase revealed new insights about the presence and interactions of Hox hydrogenase subunits and sub-complexes in vivo, providing a basis for understanding Hox assembly, maturation, and activity.

Potential Impact

This understanding will help guide future modifications to improve Hox hydrogenase activity and increase hydrogen production yields.

Year: 2013

Technical Contact: Pin-Ching Maness, pinching.maness@nrel.gov

Reference: Eckert, C.; Boehm, M.; Carrieri, D.; Yu, J.; Dubini, A.; Nixon, P. J.; Maness,
P.-C. (2012). “Genetic Analysis of the Hox Hydrogenase in the Cyanobacterium Synechocystis sp. PCC 6803 Reveals Subunit Roles in Association, Assembly, Maturation, and Function.” Journal of Biological Chemistry (287); pp. 43502–43515.

Source: NREL

The following two tabs change content below.
Do you find this article useful for you? Do you love our earth? What did you do to protect the green environment of the earth? Do you interesting on my blog and need me to post your own content or place your advertisement? Leave a comment or getting me at https://greentechbox.com/contact
Homepage » Green Energy » Green fuels » [NREL] NREL Shows How Cyanobacteria Build Hydrogen-Producing Enzyme

Leave a Reply

Your email address will not be published. Required fields are marked *